Human CYP2A6 ELISA Kit

Manufactured by Assay Genie, this Sandwich ELISA Kit is designed for the in vitro quantitative determination of Human CYP2A6 in serum, plasma, cell culture supernatants, and other biological fluids. The assay uses a double antibody sandwich format with colorimetric detection and is supplied for research use only.

Reported performance specifications include a sensitivity of 0.938ng/ml and an assay range of 1.563-100ng/ml. Precision is stated as Intra-Assay CV <8% and Inter-Assay CV <10%. Spike-and-recovery values were demonstrated for serum (range 86-103%, average 95%), EDTA plasma (range 87-103%, average 98%), and UFH plasma (range 91-105%, average 97%). Linearity was demonstrated across 1:2, 1:4, and 1:8 sample dilutions for the same three matrices.

The analyte is CYP2A6 (UniProt P11509), a cytochrome P450 family member that localises to the endoplasmic reticulum membrane. According to the supplied UniProt annotation, the enzyme exhibits high coumarin 7-hydroxylase activity, acts as the major nicotine C-oxidase, and participates in metabolic activation of aflatoxin B1 and hydroxylation of cyclophosphamide and ifosphamide. The kit should be stored at 4°C for 6 months.