Human CYP26A1 ELISA Kit

This Sandwich ELISA kit from Assay Genie is intended for the quantitative measurement of CYP26A1 in human samples using a colorimetric, double-antibody format. Validated sample types include serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. The target corresponds to Uniprot entry O43174 and the kit is grouped under the Signal Transduction research area.

Assay performance figures reported by the manufacturer include linearity across 1:2, 1:4, and 1:8 dilutions: 88-103%, 87-101%, and 87-94% for serum; 84-101%, 84-96%, and 83-99% for EDTA plasma; and 82-97%, 82-97%, and 88-100% for heparin plasma. Recovery ranges are reported as 89-99% (average 93%) for serum, 85-105% (average 91%) for EDTA plasma, and 86-100% (average 95%) for heparin plasma. Reagents are stored at 2-8°C for 12 months, with lyophilized components also stored at -20°C for extended stability.

The workflow consists of reagent equilibration and plate setup, primary incubation at 37°C for 90 minutes, biotin-labeled detection antibody binding for 60 minutes at 37°C, HRP-streptavidin incubation for 30 minutes at 37°C, TMB color development in the dark for 10-20 minutes, and a stop reaction with reading at 450 nm. CYP26A1 is a cytochrome P450 family enzyme that metabolises retinoic acid, a regulator of embryonic development, cellular differentiation, and immune response, with dysregulation implicated in cancer, inflammatory disorders, and developmental abnormalities.