Human CYP11A1/Cholesterol side-chain cleavage enzyme, mitochondrial ELISA Kit

A high-quality sandwich ELISA kit from Assay Genie is designed for the precise detection of human CYP11A1 (Cholesterol Side-Chain Cleavage Enzyme, Mitochondrial) levels. CYP11A1 is a key enzyme involved in steroid hormone biosynthesis, catalysing the conversion of cholesterol to pregnenolone, and its dysregulation has been associated with various hormonal disorders, making it a valuable biomarker for research into steroidogenesis and related conditions.

Across the Assay Genie catalogue, the kit is offered in sandwich ELISA, double antibody format and validated for use with serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples, with reactivity limited to human samples. The assay uses a colourimetric detection method and is calibrated to a detection range of 0.313-20 ng/mL with a sensitivity of 0.188 ng/mL. Performance figures provided include recovery of 85-100% (average 92%) for serum, 86-100% (average 94%) for EDTA plasma, and 88-103% (average 95%) for heparin plasma, alongside linearity ranges of 83-104% across serum, EDTA plasma and heparin plasma dilutions.

Procedure-wise, the workflow involves equilibrating reagents and TMB substrate, loading standards and samples for binding at 37°C for 90 minutes, adding a biotin-labelled detection antibody with a 60-minute incubation, binding HRP-Streptavidin (SABC) for 30 minutes, developing colour with TMB substrate in the dark for 10-20 minutes, and reading absorbance at 450 nm immediately after stop solution. The kit is stored at 2-8°C for 12 months and is supplied with components including a dismountable ELISA microplate, lyophilised standard, biotin-labelled antibody, HRP-Streptavidin conjugate, TMB substrate, sample, antibody and SABC dilution buffers, stop solution, wash buffer, plate sealer and technical manual. An alternative Assay Genie sandwich ELISA format for the same target offers a detection range of 0.312-20 ng/mL, a sensitivity of 0.133 ng/mL, intra-assay CV <10% and inter-assay CV <12%, with recovery ranges of 80-102% (serum), 81-101% (EDTA plasma) and 80-89% (heparin plasma). A further sandwich ELISA option reports a detection range of 0.312-20 ng/mL, sensitivity of 0.1 ng/mL, intra-CV of 5.3% and inter-CV of 9.6%, with serum recovery averaging 101% (range 95-107%) and plasma recovery averaging 103% (range 97-109%).