Human Cylindromatosis (CYLD) ELISA Kit

The Cylindromatosis deubiquitinase immunoassay from Assay Genie enables quantitative measurement of CYLD protein in human-derived research samples. The plate-based kit covers 96 assays and is configured for in vitro research use only, accepting tissue homogenates and cell lysates as primary input material. Researchers investigating loss-of-function mutations associated with Brooke-Spiegler syndrome, cylindroma, trichoepithelioma, and spiradenoma can use this plate to monitor protein abundance in cultured cell lines and excised tumor specimens. Each kit contains pre-coated microwells, recombinant CYLD standards, biotinylated detection antibody, streptavidin-HRP conjugate, substrate solution, and wash buffer sufficient for the full ELISA workflow. The procedure follows a standard plate-based protocol read on a microplate spectrophotometer, and shipped components remain stable for several months when stored according to the enclosed certificate of analysis. Intended applications include NF-κB pathway studies, inflammation research, oncology biomarker profiling, and characterization of deubiquitinase function in dermatologic and solid tumor model systems.