Human CYGB (Cytoglobin) ELISA Kit (HUFI04548)

This colorimetric immunoassay from Assay Genie delivers quantitative measurement of Cytoglobin in human research specimens using a microplate-based workflow. Cytoglobin (CYGB) is a hexacoordinate heme-containing globin protein broadly expressed across vertebrate tissues, including fibroblasts, hepatic stellate cells, neurons, and renal epithelium. Functional studies have linked CYGB to oxidative stress protection, nitric oxide handling, and fibrotic remodeling, and altered cytoglobin levels have been reported in hepatocellular carcinoma, renal fibrosis, and certain neurodegenerative models, making it a protein of growing biomarker interest.

The research-use kit is configured for 96 Assays per microplate and is supplied with the immunoassay reagents, recombinant protein standards, buffers, enzymatic substrate, and stop solution needed for a complete run. Sample types compatible with the workflow include human serum or plasma, cell culture supernatant, and tissue homogenate from a range of source materials. Components are intended for in vitro research applications and should not be used for diagnostic decision-making.

Unopened reagents are supplied refrigerated and should be stored refrigerated until first use, with working aliquots protected from light. Bring all materials to room temperature before beginning the run, and refer to the enclosed protocol for recommended dilutions of biofluid and tissue-lysate samples, standard curve generation, and quality-control acceptance criteria. The kit supports laboratory programs tracking CYGB dynamics in normal versus diseased human tissues, including fibrosis research, hepatology, oncology, and neuroscience studies.