Human CYFRA 21-1 PharmaGenie ELISA Kit (SBRS0469)

Assay Genie's CYFRA 21-1 PharmaGenie ELISA Kit enables quantitative measurement of the cytokeratin 19 fragment, a circulating biomarker routinely tracked in non-small cell lung cancer (NSCLC) research, treatment monitoring, and recurrence studies. The assay accepts three input matrices: serum drawn from human donors, EDTA-plasma specimens, and conditioned medium from cultured cell lines, supporting both clinical sample analysis and in vitro cell-based investigations, with a recommended 50 µL sample volume per well. Each kit ships with a pre-coated microplate, biotinylated detection antibody, lyophilized recombinant standards, streptavidin-HRP conjugate, wash buffer, and TMB substrate, with all unopened components intended for refrigerated storage at 4 degrees C. Researchers should protect the working TMB solution from light and avoid repeated freeze-thaw cycles of reconstituted standards to preserve reactivity across the full assay run.