Human Cyclophilin PharmaGenie ELISA Kit (SBRS0471)

Cyclophilin A (PPIA) is an 18 kDa immunophilin that serves as the intracellular receptor for cyclosporine A, and this Assay Genie ELISA Kit, part of the PharmaGenie reagent platform, provides a quantitative immunoassay for measuring Cyclophilin A in human research samples. The kit is configured for 96 assays with a colorimetric readout and is validated for cell culture supernatants, plasma, and serum. Cyclophilin A is investigated in research on inflammation, cardiovascular disease, viral-host interactions, and tumor biology, including studies of calcineurin/NF-AT signaling, immunosuppressant pharmacology, and extracellular cyclophilin signaling in immune and endothelial cells. Components are shipped refrigerated and should be stored at 4°C; reconstituted standards and concentrated detection reagents are best aliquoted to limit freeze-thaw cycles, with working dilutions prepared on the day of the assay. The pack includes a pre-coated microplate, biotinylated detection antibody, streptavidin-HRP conjugate, wash buffer, substrate solution, and recombinant Cyclophilin A standard for standard curve generation. Research use only.