Human Cyclophilin A (CYPA) ELISA Kit

Quantify human Cyclophilin A (CYPA) in research samples with this 96 assay colorimetric ELISA from Assay Genie. CYPA is an 18 kDa peptidylprolyl isomerase encoded by the PPIA gene, widely expressed across human tissues and best known as the intracellular binding partner of the immunosuppressant cyclosporin A.

Sample compatibility and workflow:

• Validated for human specimens including tissue homogenates, cell lysates, and cell culture supernatants
• 96 assay microplate format read on a standard plate reader
• Standard two-antibody ELISA procedure: add diluted standards and unknown samples, incubate, wash, add detection reagent, develop, and read absorbance to interpolate concentration from a standard calibration curve
• Colorimetric readout compatible with most absorbance-based plate readers in life science laboratories

Cyclophilin A accelerates protein folding by catalyzing the cis-trans isomerization of proline peptide bonds and has been investigated as a secreted signaling factor in cardiovascular disease, rheumatoid arthritis, sepsis, and several cancers, where extracellular CYPA concentrations have been reported to correlate with disease activity in published patient cohorts. It also plays documented roles in viral replication cycles, including HIV-1, hepatitis C, and several coronaviruses, making quantitative measurement in conditioned media, infected cell lysates, and tissue extracts a routine research application.