Human Cyclin Dependent Kinase 1 (CDK1) ELISA Kit

Quantitative profiling of cyclin-dependent kinase 1 in human research samples is the focus of this immunoassay kit from Assay Genie, which supports cell-cycle and oncology laboratories studying mitotic commitment and checkpoint recovery. CDK1, also historically called CDC2 and p34^cdc2, forms the catalytic core of the maturation-promoting factor (MPF) complex with cyclin B and drives the G2 to M transition. Because dysregulated CDK1 activity is a recognised feature of many proliferative disorders, this target is widely used as a biomarker in tumour-progression studies, drug-screening campaigns against small-molecule kinase inhibitors, and investigations of DNA-damage response pathways.

Supplied as a 96-assay kit for research use only, the assay accepts tissue homogenates and cell lysates as input and produces a colour-change readout on a standard microplate reader. The included reagents support a full plate run in a single batch. Common downstream applications include pairing the CDK1 readout with cyclin B co-immunoprecipitation, phospho-histone H3 staining for mitotic confirmation, or quantitative work in synchronised HeLa, U2OS, or primary fibroblast cultures released from double-thymidine or nocodazole block.