Human Cyclin B (CCNB) ELISA Kit

The Assay Genie Human Cyclin B (CCNB) ELISA Kit supports quantitative measurement of CCNB protein in research specimens. Cyclin B serves as the regulatory subunit of maturation-promoting factor (MPF), partnering with CDK1 to drive the G2/M phase transition and mitotic entry. Dysregulated CCNB expression is widely investigated in cancer biology, cell cycle checkpoint studies, and programs probing uncontrolled proliferation.

The protocol uses 100 µL sample volumes per well and accepts tissue homogenates and cell lysates. Typical applications include profiling Cyclin B across synchronized cell populations, comparing tumor versus matched normal tissue, and quantifying protein changes after pharmacologic perturbation of mitotic regulators. CCNB1 is the most-studied isoform and a recognized biomarker candidate in prostate, breast, and lung cancer research, where elevated levels correlate with poor prognosis and mitotic checkpoint failure. For research use only; not intended for diagnostic procedures.