Human CYB5D1 (Cytochrome b5 domain-containing protein 1) ELISA KIT (HUFI08201)

Assay Genie's CYB5D1 immunoassay is species-specific for human cytochrome b5 domain-containing protein 1 and quantitatively measures CYB5D1 levels in human specimens, supporting investigations into membrane-associated heme biology and endoplasmic reticulum function. The capture antibody immobilized on the microplate binds CYB5D1 from prepared samples, while a biotinylated secondary antibody and streptavidin-HRP conjugate generate a measurable colorimetric signal proportional to analyte concentration. CYB5D1 contains a conserved heme-binding cytochrome b5 fold and localizes to the endoplasmic reticulum membrane. This research use only reagent pack includes a pre-coated plate, lyophilized standard, detection antibody, and chromogenic substrate solution. Store the lyophilized components at -20 degrees C and the sealed plate at 4 degrees C protected from light to preserve antibody activity through the stated shelf life. The standard curve supports quantification across the working range, with assay precision assessed via intra-assay and inter-assay coefficient of variation values reported in the enclosed protocol insert.