Human CX40 (Connexin 40) ELISA Kit (HUES02677)

Assay Genie supplies this sandwich ELISA with a standard curve spanning roughly 0.32-20 ng/mL and a reported limit of detection near 0.126 ng/mL. The supplied lyophilized standard is reconstituted to 20 ng/mL and serially diluted to build a multi-point calibration; sample values above the highest standard should be diluted and re-assayed. The recommended sample volume is 100 µl per well, and the full protocol completes in approximately 3.5 hours at room temperature, with three wash cycles between incubation steps using the supplied 1x wash buffer. Capture and detection antibodies are provided as a pre-titrated pair, and TMB substrate is added for color development before a stop solution is introduced and absorbance read at 450 nm. Unused strips from the 96-well microplate should be resealed with the desiccant pouch and stored at 2-8°C, while reconstituted reagents retain activity for roughly two weeks under refrigeration; for longer projects, aliquot the standard and freeze at -20°C, avoiding more than three freeze-thaw cycles. Because GJA5 transcripts and protein levels are altered in hypertensive vascular beds, atrial myocyte remodeling, and congenital arrhythmia syndromes, this kit is frequently paired with immunohistochemistry on cardiac sections, western blot validation, and qPCR profiling of GJA5 against GJA1 and GJA4 expression. Intended for research use only, the kit is shipped cold and remains stable for 12 months from manufacture when stored sealed at 2-8°C, protected from light.