Human CX3CL1 (Chemokine C-X3-C-Motif Ligand 1) ELISA Kit (HUES01331)

Designed for the accurate detection of CX3CL1 (also known as fractalkine) levels in human samples, this Sandwich ELISA Kit from Assay Genie is suitable for serum, plasma and other biological fluids. CX3CL1 exists in both soluble and membrane-bound forms and plays a role in immune responses, inflammation, and neuronal communication, with dysregulation implicated in inflammatory disorders, neurodegenerative diseases, and cancer.

The colorimetric assay runs in 3 h 30 min, using a biotinylated detection antibody and an Avidin-HRP conjugate, with optical density measured at 450 nm ± 2 nm. Sensitivity is 0.1 ng/mL and the detection range is 0.16-10 ng/mL, with reported recovery of 80%-120%. The kit recognizes Human CX3CL1 with no significant cross-reactivity or interference between Human CX3CL1 and analogues was observed, and is intended for storage at 2-8°C. Research areas include Cancer, Cardiovascular, and Immunology, with UniProt ID P78423 and a sample volume of 100 μL.