Human CVB-IgM (Coxsackievirus A16-Immunoglobulin M) ELISA Kit (HUFI04721)

The Human CVB-IgM ELISA Kit from Assay Genie is a serological assay intended to detect immunoglobulin M antibodies specific to Coxsackievirus A16, one of the primary enterovirus serotypes associated with Hand, Foot, and Mouth Disease (HFMD). It supports clinical virology laboratories, infectious disease surveillance programs, and vaccine development studies focused on pediatric enterovirus outbreaks, particularly across the Asia-Pacific region where CA16 remains endemic.

IgM serology offers an early indicator of acute CA16 infection, typically appearing within the first week of symptom onset and persisting for several weeks. Researchers employ this assay to evaluate acute-phase specimens for differential diagnosis of HFMD against related pathogens such as Coxsackievirus A6 and Enterovirus 71. Each kit contains pre-coated plates, calibrated standards, detection antibodies, and substrate sufficient for 96 determinations in a single microplate run, with a recommended sample volume of 100 µL per well for unknown specimens.

Store all kit components refrigerated upon receipt to preserve antibody binding activity, protect conjugated reagents from light exposure, and avoid repeated freeze-thaw cycles of the reconstituted standards. The plate layout accommodates a standard curve alongside unknown specimens, and the complete workflow is intended for in vitro research use only, not for clinical diagnostic decision-making without further validation.