Human CRHR1/Corticotropin-releasing factor receptor 2 ELISA Kit

From Assay Genie, this double-antibody sandwich ELISA kit is intended for the quantitative detection of CRHR1 (Corticotropin-Releasing Factor Receptor 1) in human samples using a colorimetric readout. CRHR1 is a key receptor in the stress response pathway, and its dysregulation has been implicated in psychiatric disorders including depression, anxiety, and post-traumatic stress disorder, making the kit a useful tool for research into these conditions and potential therapeutic interventions.

Sensitivity is reported as 0.188 ng/mL, with a working range of 0.313–20 ng/mL. Validated sample types include serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. The kit should be stored at 2-8°C for 12 months. Recovery values were: Serum (n=5) 86–105% (average 96%), EDTA Plasma (n=5) 93–100% (average 93%), and Heparin Plasma (n=5) 91–105% (average 97%). Linearity across 1:2, 1:4, and 1:8 dilutions spanned 80–104% for serum, 85–101% for EDTA plasma, and 80–99% for heparin plasma.

The procedure involves equilibrating reagents to room temperature, incubating standards and samples at 37°C for 90 minutes, adding biotin-labeled detection antibody with a 37°C incubation for 60 minutes, binding HRP-Streptavidin (SABC) at 37°C for 30 minutes, developing with TMB substrate in the dark for 10–20 minutes, and reading absorbance at 450 nm after adding stop solution. The kit includes a dismountable ELISA microplate, lyophilized standard, biotin-labeled antibody (100X), HRP-Streptavidin conjugate (SABC, 100X), TMB substrate, sample dilution buffer, antibody dilution buffer, SABC dilution buffer, stop solution, 25X wash buffer, plate sealer, and technical manual.