Human CREB ELISA Kit

From Assay Genie, this human CREB (cAMP Response Element Binding Protein) ELISA kit is a sandwich ELISA using a double-antibody detection format with colorimetric readout, designed to quantify CREB in human biological samples for research use.

Validated sample types include serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. The kit's standard range is 0.313-20 ng/mL with a sensitivity of 0.188 ng/mL. Storage is at 2-8°C for 12 months. The associated UniProt identifier is P16220, and listed synonyms include CREB, active transcription factor CREB, cAMP responsive element binding protein 1, CREB-1, cyclic AMP-responsive element-binding protein 1, and MGC9284.

The procedure uses a biotin-labeled detection antibody with HRP-Streptavidin and TMB substrate, with absorbance read at 450 nm. Reported linearity for serum (n=5) at 1:2, 1:4, and 1:8 dilutions is 89-101%, 87-98%, and 88-99% respectively; EDTA plasma (n=5) shows 91-104%, 88-99%, and 89-100%; heparin plasma (n=5) shows 90-105%, 87-96%, and 89-95%. Recovery ranges are 87-102% (average 95%) for serum, 88-102% (average 97%) for EDTA plasma, and 88-102% (average 97%) for heparin plasma. CREB is described as a key transcription factor involved in neuronal plasticity, memory formation, and cell survival, with dysregulation linked to cancer, neurological disorders, and psychiatric conditions.