Human Creatine Kinase, Brain (CKB) ELISA Kit

Quantitative measurement of human brain-type creatine kinase (CKB) underpins research into cellular energy metabolism, neurological disease, and oncology. CKB catalyzes the reversible transfer of a phosphoryl group between ATP and phosphocreatine, regenerating ATP in tissues with high or fluctuating energy demand, including brain and skeletal muscle. Dysregulated CKB expression and release into circulation have been examined as a biomarker in ischemic stroke, traumatic brain injury, neurodegenerative conditions, and several solid tumors, where elevated isozyme activity has been linked to disease progression and poor prognosis.

From Assay Genie, this ELISA Kit is configured for 96 assays and validated for human specimens. Acceptable sample types span tissue homogenates, cell lysates, cell culture supernatants, and fluid specimens collected from human donors. The microplate-based protocol produces an absorbance read-out that researchers quantify against a standard curve. Core laboratories studying acute central nervous system damage, rhabdomyolysis, or tumor-associated metabolic reprogramming apply this kit to patient-derived and experimental material. Reagents are supplied in a single kit box; store refrigerated and equilibrate working dilutions to room temperature before initiating the assay.