Human Corticotropin Releasing Hormone Receptor 2 (CRHR2) ELISA Kit

Built to quantify corticotropin-releasing hormone receptor 2 (CRHR2) in human samples, this 96-assay ELISA kit from Assay Genie supports research into G-protein coupled receptor signaling, stress-axis biology, and cardiovascular or gastrointestinal receptor studies. The assay accepts tissue homogenates and cell lysates as input, making it appropriate for in vitro work on cultured cells and for ex vivo tissue analysis.

CRHR2 is a class B GPCR that binds CRH and the urocortin peptides and signals primarily through Gs, raising intracellular cAMP. It is expressed as two main splice variants, CRHR2α and CRHR2β, with distinct tissue distributions: the α form predominates in the central nervous system, while the β form is more abundant in peripheral tissues such as the heart, skeletal muscle, and the gut. Researchers use quantitative CRHR2 measurement to track receptor expression under different physiological and pharmacological conditions, including chronic stress models, inflammation studies, and evaluation of candidate CRHR2 antagonists.

The kit is supplied in a 96-assay configuration and is intended for research use only. Quantification uses a standard curve-based approach compatible with standard microplate readers. Researchers should consult the package insert for sample preparation, dilution, and storage conditions specific to their experimental design.