Human Corticotropin Releasing Hormone Receptor 1 (CRHR1) ELISA Kit

Receptor-level quantification of human Corticotropin Releasing Hormone Receptor 1 (CRHR1) is supported by this microplate-based immunoassay from Assay Genie, configured for a colorimetric readout. CRHR1 is a class B G protein-coupled receptor that mediates the biological actions of corticotropin-releasing hormone and related urocortin peptides, serving as a central regulator of the hypothalamic-pituitary-adrenal (HPA) axis and the neuroendocrine response to stress. The kit finds application across neuroscience, receptor pharmacology, and endocrinology research, where protein-level expression data are used to complement transcript-level studies.

Compatible with tissue homogenates and cell lysates derived from human sources, the assay accommodates work on primary cultures, established cell lines, and dissected tissues relevant to investigations of mood regulation, anxiety biology, addiction mechanisms, and inflammatory signaling. Each kit provides the essential reagents required to perform the ELISA workflow, with a protocol that integrates into standard plate-reader-based laboratory routines. Researchers studying CRHR1 in the context of HPA axis dysregulation or stress-related pathology can use this kit to generate reproducible receptor quantification data from limited sample material.

The human specificity of the assay aligns with translational research focused on psychiatric disorders, neurodegenerative conditions, and endocrine investigations, where CRHR1 expression patterns offer insight into disease mechanisms and potential therapeutic targets. Corticotropin-releasing hormone signaling through CRHR1 activates adenylate cyclase and elevates intracellular cAMP, coupling receptor engagement to downstream physiological and behavioral responses including catecholamine release, glucocorticoid secretion, and modulation of synaptic plasticity.