Human COR (Cortisol) ELISA Kit (HUFI07675)

Quantitative determination of cortisol in human specimens is supported by this competitive enzyme-linked immunosorbent assay from Assay Genie. The 96-assay kit uses a colorimetric readout with horseradish peroxidase-based enzymatic chemistry, producing absorbance changes that correlate with cortisol concentration quantified against a standard curve.

Cortisol, the principal glucocorticoid secreted by the adrenal cortex, governs stress response, glucose metabolism, and circadian rhythm. Investigators in endocrinology, psychobiology, sleep research, and metabolic disease use this kind of competitive immunoassay to quantify circulating cortisol in research cohorts. The kit is intended for in vitro research use only and is not approved for diagnostic procedures.

Packaging includes a pre-coated microplate, calibration standards, assay buffer, detection antibody, and substrate solution sufficient for 96 determinations. Most protocol steps are performed at room temperature, with the kit components stored refrigerated between uses. Researchers should consult the enclosed manual for specific sample preparation guidance, dilution schemes for serum or plasma specimens, and recommended plate-reading wavelength.