Human Complement Component 5 (C5) ELISA Kit

Complement C5, the cleavage of which generates the anaphylatoxin C5a and the membrane-attack-complex initiator C5b, can be quantified in human specimens using the Assay Genie Human Complement Component 5 (C5) ELISA Kit, a colorimetric microplate immunoassay intended for in vitro research use only. C5 sits at a pivotal point in the complement activation cascade, so circulating C5 concentrations are widely tracked in studies of innate immunity, autoimmune disease, transplant biology, sepsis, and complement-driven hemolytic disorders.

The kit is supplied in a microplate format with all components required to build a standard curve and quantify native human C5. Unopened reagents are stored refrigerated and protected from light to maintain conjugate activity, while reconstituted standards should be aliquoted to limit repeated freeze-thaw cycles. The assay is validated for human reactivity and supports workflows ranging from single-specimen biomarker checks to moderate-throughput cohort screening.

Typical applications include pharmacodynamic monitoring of C5-directed therapeutics such as complement inhibitors, mechanistic complement activation studies in inflammatory patient cohorts, biomarker surveillance in autoimmune groups, and basic research into innate immune signaling. The microplate format accommodates longitudinal time-course sampling and paired treatment-versus-control study designs commonly used in immunology and translational research laboratories.