Human Complement 1q (C1q) ELISA Kit

Measuring circulating Complement component 1q (C1q) is central to classical pathway immunology research, and this ELISA-format kit from Assay Genie provides a quantitative immunoassay workflow for that purpose. C1q acts as the recognition subunit that initiates the complement cascade after binding antibody-antigen immune complexes, driving opsonization, inflammatory signaling, and clearance of pathogens and apoptotic cells. Altered serum C1q levels are documented in autoimmune disease, with hereditary or acquired C1q deficiency strongly linked to systemic lupus erythematosus and lupus nephritis. Researchers studying complement activation in glomerulonephritis, transplant rejection, ischemic injury, neurodegeneration, and infectious disease also rely on quantitative C1q measurements to characterize immune status.

Reagents are supplied in a microplate kit format configured for 96 individual determinations per plate. Each well plate is pre-coated with anti-C1q capture antibodies and the assay incorporates a labelled detection reagent that generates a measurable, analyte-proportional signal. Calibrators prepared from recombinant C1q are run as a serial dilution to build a standard curve, enabling interpolation of unknowns. The procedure accepts serum, tissue homogenates, cell lysates, and conditioned media from cultured cells as input material.

Intended for research use only, this in vitro kit supports ELISA-based workflows in immunology, nephrology, rheumatology, and inflammation studies. Typical applications include screening patient cohorts for C1q deficiency, monitoring complement consumption during disease flares, and evaluating treatment response in autoimmune or immune-complex-mediated conditions.