Human Colony Stimulating Factor 3, Granulocyte (GCSF) ELISA Kit

Granulocyte Colony Stimulating Factor 3 (GCSF, also called CSF3) is a 174-residue glycoprotein cytokine that drives neutrophil proliferation, differentiation, and release from bone marrow. This Assay Genie quantitative immunoassay uses paired antibodies in a sandwich configuration, with a colorimetric readout read at 450 nm against a standard curve to quantify GCSF in serum, plasma, tissue homogenates, cell lysates, and cell culture supernatants.

The kit covers a working detection range of 15.6 to 1000 pg/mL, with a sensitivity near 5.8 pg/mL and a 100 µl sample volume per well. Between runs, kit components are stored refrigerated at 2-8 degrees C while standards and the coated microplate are kept frozen at -20 degrees C for longer-term stability, with limited freeze-thaw cycles recommended to preserve antibody binding activity. Investigators studying inflammation, sepsis, neutropenia recovery after chemotherapy-induced myelosuppression, and stem cell mobilization rely on this assay to track circulating GCSF concentrations in clinical and preclinical cohorts, with the human-specific capture and detection antibodies showing no significant cross-reactivity with related murine cytokines.