Human Collectin-11 (COLEC11) ELISA Kit

From Assay Genie, this colorimetric Sandwich ELISA Kit is designed for the precise measurement of Collectin-11 (COLEC11, also known as CL-K1 or Collectin kidney protein 1) levels in human samples. The assay is reactive with human samples and validated for use with serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids, with specificity for natural and recombinant human Collectin-11. Intended for research use only, the kit is classified under the Cell Biology research area and targets a secreted protein involved in innate immunity, pathogen recognition and clearance, with dysregulation linked to various inflammatory and autoimmune diseases.

Reported performance characteristics include a detection range of 78-5000pg/mL, a sensitivity of 39pg/mL, an intra-assay CV of 6.7%, and an inter-assay CV of 9.8%. Linearity spans 85-120% across serum, EDTA plasma and heparin plasma at dilutions from 1:2 to 1:16. Recovery averages 106% (range 100-112%) for serum and 108% (range 102-114%) for plasma. Functionally, Collectin-11 is a calcium-dependent lectin that binds sugars including fucose and mannose (with a higher affinity for fucose), recognizes a subset of fucosylated glycans and lipopolysaccharides (LPS), and has been implicated in innate immunity, apoptosis and embryogenesis, where it is described as a guidance cue for neural crest cell migration.