Human Collagen Type XV (COL15) ELISA Kit

Collagen Type XV (COL15) is a basement membrane-associated homotrimeric collagen of the multiplexin subfamily, structurally related to endostatin-yielding collagen XVIII. The protein localizes to vascular smooth muscle, endothelial cells, skeletal muscle fibers, and the basement membrane zone, where it contributes to microvascular stability and extracellular matrix organization. Investigators profile COL15 levels when studying tumor angiogenesis, myocardial fibrosis, diabetic microvascular complications, and muscular dystrophy models.

This quantitative immunoassay from Assay Genie is configured for human samples and provides a sensitive means of measuring native COL15 protein. The kit ships with a pre-coated microtitre plate, lyophilised recombinant standards, biotinylated detection reagent, streptavidin-HRP conjugate, wash buffer, substrate solution, and stop reagent sufficient for one plate. Reconstituted standards should be frozen at -20 degrees C in single-use aliquots to prevent freeze-thaw degradation, while working dilutions may be refrigerated at 4 degrees C for short-term use. Each microplate well accommodates a 100 uL sample or standard volume. The procedure is validated for research use only and supports applications in cardiovascular biology, oncology biomarker discovery, and connective tissue research.