Human Collagen Type IV Alpha 3 (COL4a3) ELISA Kit

The Assay Genie COL4a3 immunoassay enables quantitative measurement of Collagen Type IV Alpha 3 in tissue homogenates and cell lysates. This 96-assay kit supports research into glomerular basement membrane biology, Alport syndrome, and related renal pathologies where COL4a3 chain abundance serves as a biomarker of interest.

Each kit contains a pre-coated microplate, calibration standards, detection reagent, and assay buffer sufficient for completing 96 individual reactions. The procedure relies on a standard curve generated from serially diluted reference material, with absorbance values captured on a compatible plate reader. Researchers frequently pair results with western blot or immunohistochemistry follow-up when characterizing COL4a3 distribution in patient biopsies or experimental animal tissue.

Unopened components should be kept refrigerated and shielded from light; allow reagents to reach room temperature before initiating the protocol. The kit is designated for research use only and has not been validated for clinical diagnostic application.