Human Collagen and calcium-binding EGF domain-containing protein 1 (CCBE1) ELISA Kit

The Human CCBE1 quantitative immunoassay from Assay Genie enables researchers to measure collagen and calcium-binding EGF domain-containing protein 1 across human-derived research specimens. CCBE1 is a secreted factor best characterized for its role in lymphatic vessel formation, and altered expression has been linked to primary lymphedema, Hennekam syndrome, and selected tumor microenvironments, making accurate quantitation relevant to vascular biology, rare-disease, and oncology research programs.

The kit is supplied in a 96 assays configuration and includes a pre-coated microplate, capture and detection antibodies, recombinant human CCBE1 standard, substrate, wash buffer, and stop solution. A 50 µL sample volume is added per well, followed by incubation with biotinylated detection reagent and streptavidin-HRP conjugate. Color development is read on a standard microplate reader, and CCBE1 concentrations are interpolated from the standard curve generated on the same plate.

Compatible input matrices span human serum, EDTA-plasma and heparin-plasma fractions, tissue homogenates, cell lysates, and clarified cell culture supernatants. Reagent components should be stored refrigerated at 4 °C and protected from light; the pre-coated plate and lyophilized standards remain stable through the kit expiration when handled according to the supplied protocol. For research use only; not intended for diagnostic procedures.