Human COL9A3 ELISA Kit

A sandwich ELISA kit from Assay Genie designed for the quantitative measurement of human COL9A3 (Collagen alpha-3, IX chain; UniProt Q14050) in serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. The double-antibody sandwich format is paired with a colorimetric readout, in which TMB substrate is developed and absorbance is read at 450 nm after stop solution is added.

Reported performance characteristics include a sensitivity of 18.75 pg/mL and a working range of 31.25–2000 pg/mL. Dilutional linearity in serum (n=5) spans 89–104% at 1:2, 89–104% at 1:4, and 87–103% at 1:8; EDTA plasma (n=5) gives 84–97%, 82–101%, and 88–101%; heparin plasma (n=5) yields 80–94%, 91–97%, and 81–92%. Spike-and-recovery averages are 93% for serum (range 86–100%), 96% for EDTA plasma (range 86–104%), and 95% for heparin plasma (range 89–103%). Unopened reagents are stored at 2–8°C for 12 months. The workflow involves equilibrating reagents to room temperature, primary incubation at 37°C for 90 minutes, biotinylated detection antibody incubation for 60 minutes, HRP-Streptavidin (SABC) for 30 minutes, TMB colour development in the dark for 10–20 minutes, and immediate absorbance reading at 450 nm.

Supplied components include a dismountable ELISA microplate, lyophilized standard, biotin-labeled detection antibody (100X concentrate), HRP-Streptavidin conjugate (SABC, 100X), TMB substrate, sample dilution buffer, antibody dilution buffer, SABC dilution buffer, stop solution, 25X wash buffer, plate sealers, and a technical manual. The kit is intended for cell biology research into COL9A3, a structural component of type IX collagen linked to cartilage integrity and disorders such as osteoarthritis and intervertebral disc degeneration.