Human COL2/CollagenII ELISA Kit

Developed for the quantitative measurement of collagen II in human biological samples, this Sandwich ELISA Kit from Assay Genie is intended for research applications. The assay employs a double-antibody sandwich format with colorimetric detection and is validated for use with serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. The kit delivers a sensitivity of 28.125 pg/mL across a standard curve range of 46.875-3000 pg/mL.

Recovery in serum (n=5) is reported between 93-102% with an average of 96%; EDTA plasma (n=5) between 88-99% with an average of 95%; and heparin plasma (n=5) between 89-101% with an average of 95%. Reagents are stored at 2-8°C for 12 months. The kit supports studies of cartilage-related disorders such as osteoarthritis, where changes in collagen II levels are of investigative interest.