Human COL1A1/Collagen I alpha 1 ELISA Kit

A Sandwich ELISA from Assay Genie designed for the quantitative detection of Human COL1A1 (Collagen Type I Alpha 1) in serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. The kit uses a colorimetric detection method with a double-antibody sandwich format and reports a sensitivity of 0.188 ng/mL across a working range of 0.313-20 ng/mL.

Validated performance includes linearity across dilutions (1:2, 1:4, 1:8) in serum (89-104%, 92-101%, 80-95%), EDTA plasma (85-93%, 85-98%, 80-95%), and heparin plasma (87-104%, 84-99%, 84-96%), with recovery rates averaging 94% for serum, 93% for EDTA plasma, and 91% for heparin plasma. Reagents are stored at 2-8°C for 12 months.

Collagen type I alpha 1 is a key component of collagen type I, the most abundant collagen in the human body, and plays a vital role in wound healing, bone formation, and skin elasticity. Dysregulation of collagen type I alpha 1 levels is associated with diseases including osteoporosis, arthritis, and fibrosis, making it a valuable biomarker for tissue remodeling and regeneration research.