Human COL14 (Collagen Type 14) CLIA Kit (HUES00527)

Assay Genie's CLIA Kit for Human COL14 (Collagen Type 14) is a sandwich chemiluminescence immunoassay designed for the precise measurement of collagen type 14 levels in human samples. The kit offers high sensitivity and specificity, supporting research into the role of this extracellular matrix component in tissue development, wound healing, and cell adhesion, and its potential links to conditions including fibrosis, arthritis, and skin disorders.

The assay uses a pre-coated microplate, biotinylated detection antibody, and HRP-conjugated streptavidin, with a chemiluminescent substrate producing light intensity proportional to target concentration. Sensitivity is 18.75 pg/mL with a detection range of 31.25-2000 pg/mL and recovery of 80%-120%. Samples include serum, plasma and other biological fluids with a recommended volume of 100 μL. The total assay time is 3 h 30 min.

The kit is validated for human samples with no significant cross-reactivity or interference between Human COL14 and analogues observed. It is intended for research use within the signal transduction field (UniProt ID Q05707), with components stored at 2-8°C/-20°C.