Human Cocaine- and amphetamine-regulated transcript protein (CARTPT) ELISA Kit

A Sandwich ELISA Kit from Assay Genie designed for the quantitative detection of human Cocaine- and amphetamine-regulated transcript protein (CARTPT). The colorimetric assay is reactive with human samples and is intended for research use only, providing a tool for studies exploring appetite regulation, energy balance, reward pathways and addiction-related processes.

The kit reports a detection range of 78-5000pg/mL with a sensitivity of 33pg/mL. Reported precision values are an intra-assay CV of 5.2% and an inter-assay CV of 8.3%. Linearity has been evaluated in serum, EDTA plasma and heparin plasma across serial dilutions, with recovery averaging 88% (range 82-94%) in serum and 90% (range 84-96%) in plasma. CARTPT is described as a satiety factor closely associated with the actions of leptin and neuropeptide Y, inhibiting both normal and starvation-induced feeding and promoting neuronal development and survival in vitro.

Validated sample types include serum, plasma, tissue homogenates, cell culture supernatants and other biological fluids. The kit recognises natural and recombinant human CARTPT, and the target is listed as a secreted protein associated with obesity in the UniProt and NCBI entries.