Human CML (Carboxymethyl Lysine) ELISA Kit (HUFI04758)

Carboxymethyl Lysine (CML) is one of the best-characterized advanced glycation end-products (AGEs), formed when reducing sugars react non-enzymatically with amino groups on proteins, lipids, and nucleic acids. The Assay Genie CML ELISA Kit is a competitive immunoassay supplied in a 96 Assays pack with a colorimetric readout for quantitative measurement of CML accumulation in human clinical samples.

Elevated CML levels are studied as biomarkers of chronic oxidative stress and long-term glycemic burden, and they appear in research on diabetic complications, atherosclerotic cardiovascular disease, and chronic kidney disease. The kit is intended for research use only, supporting studies that explore dietary AGE intake, renal clearance of AGE-modified proteins, and the contribution of AGE accumulation to inflammatory signaling pathways.

The competitive format pairs a pre-coated plate with HRP-based detection and a substrate solution that is read on a standard microplate reader. Each kit provides reagents sufficient for 96 individual determinations, with all buffers, plates, and detection components packaged together for a single-use workflow. Reconstituted and working reagents should be stored refrigerated and kept away from light to preserve enzymatic activity throughout the run.