Human Cluster Of Differentiation 40 Ligand (CD40L) ELISA Kit

Quantitative measurement of soluble CD40 Ligand (sCD40L, also called CD154) — a 39 kDa type II transmembrane glycoprotein of the TNF superfamily that mediates T cell costimulation of B cells, drives immunoglobulin class switching, and supports germinal center formation — is enabled by this immunoassay from Assay Genie. sCD40L is widely used in translational research as a circulating biomarker of platelet activation, vascular endothelial dysfunction, and adaptive immune engagement, and is studied in the context of atherosclerosis, acute coronary syndromes, systemic lupus erythematosus, transplant rejection, and chronic inflammatory disease.

The kit is configured for 96 assays and uses a colorimetric read-out compatible with standard microplate readers. Validated sample matrices include plasma, serum, tissue homogenates, and cell lysates, allowing investigators to move between clinical plasma specimens, in vitro cell culture supernatants, and animal-model tissue work without switching platforms.

Intended for research use only, the assay supports applications including flow cytometry–adjacent biomarker studies, vascular inflammation research, autoimmune disease profiling, and vaccine adjuvant investigations. Lot-specific standard curves, recommended dilution guidance, and a complete step-by-step protocol are included in the package insert.