Human Cluster Of Differentiation 226 (CD226) ELISA Kit

CD226 (cluster of differentiation 226), also known as DNAM-1 (DNAX accessory molecule-1), is a 65 kDa type I transmembrane glycoprotein belonging to the immunoglobulin superfamily. It is constitutively expressed on natural killer (NK) cells, CD4+ and CD8+ T lymphocytes, monocytes, and a subset of B cells, where it mediates adhesion to target cells through its ligands CD112 (Nectin-2) and CD155 (poliovirus receptor, PVR). Engagement of CD226 triggers NK cell and cytotoxic T cell activation, granule polarization, and target cell lysis, making the molecule of substantial interest in tumor immunology, viral infection research, and autoimmune disease studies. Quantitative measurement of CD226 protein levels supports investigations of lymphocyte signaling, ligand-receptor engagement, and effector cell cytotoxicity in vitro and in disease-relevant model systems.

The Assay Genie Human CD226 ELISA kit is supplied as a complete reagent set including a pre-coated microplate, biotinylated detection antibody, streptavidin-HRP conjugate, recombinant protein calibrators, wash buffer, and substrate solution, providing enough material for 96 individual determinations per run. The procedure follows a sequential incubation protocol that yields a colorimetric readout measurable on a standard microplate reader, with the full assay generally completed within several hours of sample addition. Recommended specimen types include serum samples, plasma-derived specimens, tissue homogenates, and cell culture supernatants. The kit is intended for research use only and is not validated for diagnostic, prognostic, or therapeutic applications.