Human Cluster Of Differentiation 200 (CD200) ELISA Kit

A type I transmembrane glycoprotein broadly expressed across thymocytes, B and T lymphocytes, neurons, endothelial cells, and several tumor lineages, Cluster of Differentiation 200 (CD200), also known as OX-2, engages the inhibitory receptor CD200R on myeloid and lymphoid populations to deliver tolerance signals that dampen innate immune activation. Quantifying circulating CD200 has become a valuable readout in studies of autoimmune disease, graft-versus-host response, multiple myeloma, chronic lymphocytic leukemia, and chronic inflammatory conditions.

The Assay Genie two-antibody quantitative immunoassay measures soluble CD200 in human specimens, with colorimetric readout proportional to analyte concentration. Validated matrices include serum fractions, heparin plasma fractions, EDTA plasma fractions, and cell culture supernatant samples, making the kit applicable to immunology, oncology, and transplantation research. Each kit supplies a pre-coated microplate in a sealed foil pouch with desiccant, biotinylated detection antibody, streptavidin-HRP conjugate, wash buffer concentrate, substrate solution, and stop reagent. The protocol accommodates a 50 µL sample volume per well, and reconstituted standards or strips remain stable when stored refrigerated while working dilutions should be kept frozen and protected from light to preserve immunoreactivity through repeated freeze-thaw cycles. Intended for research use only, the kit supports batch analysis in biomarker discovery laboratories.