Human Cluster Of Differentiation 14 (CD14) ELISA Kit

The CD14 immunoassay from Assay Genie measures human cluster of differentiation 14, a 55 kDa glycosylphosphatidylinositol-anchored pattern recognition receptor that operates alongside TLR4 and MD-2 in detecting bacterial lipopolysaccharide. Built on a paired-antibody capture format, the 96-assay configuration supports in vitro research into innate immunity, monocyte and macrophage activation pathways, and soluble CD14 as a biomarker in sepsis, autoimmune conditions, and inflammatory disorders.

The kit accepts cell lysates along with several human-derived sample matrices and serves pharmacodynamic studies of immunomodulatory drugs, characterization of patient cohorts with chronic inflammation, and time-course experiments on LPS-stimulated monocyte cultures. Components include a pre-coated microplate, biotinylated detection reagent, enzyme conjugate, calibrator standards, and substrate; the assay is intended for research use only and is not validated for clinical diagnostic procedures.

Unopened reagents remain stable when refrigerated, while reconstituted standards benefit from aliquoting to limit freeze-thaw exposure. The microplate layout supports duplicate measurements of multiple samples in parallel with a full standard curve, balancing throughput with the precision needed for low-abundance analytes such as circulating sCD14.