Human CLPS (Colipase) ELISA Kit (HUFI07960)

Built by Assay Genie for translational and basic-research laboratories, this CLPS ELISA Kit is a quantitative sandwich immunoassay that quantifies human colipase, the small pancreatic cofactor that tethers lipase to the lipid-water interface during triglyceride hydrolysis. The pre-coated microplate format supports standard curves spanning detection range 1.25 to 80 ng/mL, with sensitivity 0.42 ng/mL calculated as 2SD above the zero standard. An intra-assay CV of 5.6% and inter-assay CV of 10.9% across plasma controls underpin reproducible run-to-run performance, and spike-recovery in human plasma averages 100% (range 95 to 107%). The protocol uses a 50 uL sample volume per well, biotinylated detection antibody, streptavidin-peroxidase conjugate, and a TMB chromogen read at 450 nm. The 96-well kit accommodates serum and plasma specimens from cohorts investigating pancreatic exocrine insufficiency, cystic fibrosis-related fat malabsorption, chronic pancreatitis, and lipid-metabolism disorders, and is supplied as single-use components for research use only. Unopened reagents should be stored refrigerated at 2-8 degrees C in the sealed foil pouch to preserve calibration; avoid freeze-thaw cycles of the lyophilized standard, and equilibrate all components to room temperature before each run.