Human CLDN14 (Claudin 14) ELISA Kit (HUES01891)

The Claudin 14 protein encoded by the CLDN14 gene is a tight junction component predominantly studied in auditory and renal research, where it regulates paracellular ion permeability in the inner ear and the thick ascending limb of the loop of Henle. This sandwich ELISA from Assay Genie enables quantitative measurement of human Claudin 14 in serum, plasma, and other biological fluids using a colorimetric readout at 450 nm.

• Format: 96-well microplate with pre-coated capture antibodies
• Sample volume: typically 100 µL per well
• Detection method: colorimetric, compatible with standard plate readers
• Storage: 2-8 °C for the unopened kit; aliquot and freeze at -20 °C for long-term storage; avoid repeated freeze-thaw cycles
• Application: research use only, in vitro quantification of Claudin 14 in human samples

Investigate biomarkers linked to autosomal recessive non-syndromic hearing loss (DFNB29) and disorders of calcium handling such as hypercalciuria and nephrolithiasis. The assay supports serum, plasma, and tissue homogenate workflows, and includes standards, detection antibody, and TMB substrate needed to generate a quantitative standard curve.