Human Claudin 5 (CLDN5) ELISA Kit

Claudin-5 (CLDN5) is the principal tight-junction transmembrane protein of vascular endothelium and the defining structural element of the blood-brain barrier, where it controls paracellular permeability and maintains the selective seal between circulating blood and the central nervous system. The Assay Genie CLDN5 ELISA Kit provides quantitative measurement of claudin-5 levels in human-derived samples, supporting research into BBB dysfunction, ischemic stroke, multiple sclerosis, glioblastoma angiogenesis, and inflammatory vascular disease. Investigators studying endothelial barrier breakdown, drug transport across the CNS endothelium, and tumour-microenvironment permeability rely on claudin-5 quantification as a biomarker of junctional integrity. The kit is supplied as a 96-assay microplate format containing pre-coated wells, detection antibody, calibrated standards, and substrate solution sufficient to generate a standard curve across the expected physiological range. Reagent components should be stored refrigerated between uses, protected from light, and allowed to equilibrate to room temperature before initiating the assay protocol. Each kit is configured as a single-use immunoassay reagent set intended for research use only; reconstituted standards should be aliquoted into tubes to limit freeze-thaw cycles and preserve antibody activity through the stated shelf life.