Human Chymotrypsinogen B2 (CTRB2) ELISA Kit

This quantitative immunoassay from Assay Genie targets human chymotrypsinogen B2, a zymogen secreted by pancreatic acinar cells as the inactive precursor of chymotrypsin B. Investigators studying pancreatic exocrine function, gastrointestinal proteolysis, or hereditary pancreatitis biology can use it to quantify CTRB2 levels. The kit ships with a pre-coated microplate, lyophilized and liquid reagents, and a recombinant standard calibrator.

Each kit provides 96 individual assays, supporting a full standard curve alongside duplicate unknowns. Unopened components arrive on cold packs and should be stored refrigerated until first use; opened vials and reconstituted standards benefit from being aliquoted and frozen for long-term storage to protect the working reagents from degradation through repeated freeze-thaw cycles. Allow all components to equilibrate at room temperature before the run, and keep pre-coated plates in their sealed pouch with desiccant to prevent condensation on the well surface.

The plate-based immunoassay workflow fits standard laboratory ELISA readers and accommodates cell culture supernatants, tissue lysates, and clarified biofluids. Research use only, the kit is supplied as a complete pack with a coated plate, biotinylated detection reagent, HRP conjugate, wash concentrate, diluent, TMB substrate, stop solution, and standard in individual vials and ampoules for single-use handling.