Human Cholinergic Receptor, Nicotinic, Alpha 5 (CHRNa5) ELISA Kit

The CHRNa5 target encodes the alpha-5 subunit of neuronal nicotinic acetylcholine receptors, a ligand-gated ion channel implicated in nicotine dependence, lung cancer susceptibility, and neuropsychiatric conditions including schizophrenia and cognitive disorders. This two-antibody immunoassay from Assay Genie enables quantitative measurement of the receptor subunit across tissue homogenates and cell lysates, supporting studies in pulmonary oncology, addiction neuroscience, and pharmacogenomics.

Each kit supplies pre-coated microplate wells, biotinylated detection reagent, streptavidin conjugate, calibrated recombinant standards, and wash buffer sufficient for 96 assays run in duplicate. Researchers typically load serial standard dilutions to construct a multi-point calibration curve, then interpolate unknown sample concentrations against it. The kit is intended for research use only and is compatible with standard absorbance plate readers.

Store unopened components refrigerated and protect from light; reconstituted working standards and detection antibody should be aliquoted and frozen to limit freeze-thaw cycles. Sample preparation generally involves homogenization in cold lysis buffer containing protease inhibitors, followed by centrifugation at 10,000 x g to clear debris before loading the wells.