Human Choline O-acetyltransferase (CHAT) ELISA Kit

Designed for the precise detection of Choline O-acetyltransferase (CHAT) levels in human samples, this Sandwich ELISA Kit from Assay Genie delivers accurate and reproducible results for a range of research applications. CHAT is an enzyme that plays a critical role in the synthesis of the neurotransmitter acetylcholine, which is essential for proper nerve function and communication.

The kit shows a detection range of 0.156-10ng/mL and a sensitivity of 0.088ng/mL, with an intra-assay CV of 7.6% and an inter-assay CV of 8.5%. Linearity has been validated in serum, EDTA plasma, and heparin plasma samples. Recovery averages 102% (range 96-108%) for serum and 104% (range 98-110%) for plasma. It is suitable for use with serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids, with specificity for natural and recombinant human CHAT.

CHAT catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses. Dysregulation of CHAT has been implicated in neurological disorders such as Alzheimer's disease, making it a valuable biomarker for studying these conditions and potential therapeutic interventions. For research use only.