Human Cholesterol-25-Hydroxylase (CH25H) ELISA Kit

Cholesterol-25-Hydroxylase (CH25H) is a membrane-associated oxygenase that converts cholesterol into 25-hydroxycholesterol, an oxysterol implicated in macrophage activation, innate antiviral immunity, and chronic inflammatory signaling. The Assay Genie CH25H ELISA Kit delivers a quantitative in vitro immunoassay for research use only, measuring human CH25H levels across diverse experimental samples with a colorimetric microplate readout.

This 96-assay format supplies pre-coated plate wells, calibrated recombinant protein standards, a CH25H-specific detection antibody, and substrate solution, supporting laboratory studies of lipid metabolism, sterol biosynthesis, and immune regulation pathways. A printed protocol accompanies each kit, and reagents are sufficient to process multiple sample types within a single experimental run.

Applications include investigation of atherosclerosis, viral infection response, metabolic syndrome, and oxysterol biology in tissue homogenates and cell culture supernatant preparations. Academic and pharmaceutical research groups focused on cholesterol pathway targets, inflammatory disease mechanisms, and biomarker discovery can integrate this ELISA into mechanistic studies of CH25H enzyme function and its transcriptional regulation under metabolic challenge.