Human Chloride Intracellular Channel Protein 1 (CLIC1) ELISA Kit

The CLIC1 protein targeted by this kit is a 27 kDa chloride intracellular channel involved in regulating membrane potential, cell volume, and acidifying organelle lumens. In cancer research, elevated CLIC1 expression has been documented in glioma, gastric cancer, and hepatocellular carcinoma, where it contributes to proliferation, migration, and invasion pathways, making accurate quantification valuable for biomarker and pathway studies. Each Assay Genie kit is supplied with a pre-coated 96-well microplate, capture and detection antibodies, standards, and buffers sufficient for 96 determinations.

Researchers apply this quantitative ELISA to tissue homogenates, cell lysates, and other biological fluids, with each well loaded using a 100 uL sample volume to generate a standard curve. The assay uses a human-specific capture antibody with no significant cross-reactivity with rat or mouse CLIC1 orthologs, supporting clear interpretation in translational studies. Store reagents refrigerated until first use and avoid repeated freeze-thaw cycles to preserve antibody activity. For research use only; not intended for diagnostic procedures.