Human Chloride Channel Accessory 1 (CLCA1) ELISA Kit

This quantitative immunoassay from Assay Genie quantifies CLCA1, a calcium-activated chloride channel accessory protein expressed in airway and intestinal mucosal epithelia. CLCA1 regulates chloride flux across epithelial barriers and is upregulated in goblet cells during Th2-driven inflammation, making it a target of interest in asthma, COPD, cystic fibrosis, and ulcerative colitis research.

The kit arrives as a complete reagent set including a pre-coated microplate, capture and detection antibodies, calibrated reference standards, substrate solution, and stop reagent. Researchers typically load 100 uL of diluted sample per well, with tissue homogenates and cell lysates prepared in cold lysis buffer supplemented with protease inhibitors. Unopened kits are shipped refrigerated and components should be stored at -20 degrees C for long-term use; the reconstituted standard should be aliquoted to avoid repeated freeze-thaw cycles.

Each kit contains sufficient reagents for one full microplate of samples, standards, and controls run in duplicate. For research use only; not intended for diagnostic procedures.