Human Chitinase-3-like protein 1 (CHI3L1) ELISA Kit

Manufactured by Assay Genie, this Sandwich ELISA is intended for the precise quantification of human Chitinase-3-like protein 1 (CHI3L1), also referred to as YKL-40, 39 kDa synovial protein, Cartilage glycoprotein 39, and CGP-39. The kit supports research into inflammation and tissue remodeling, where elevated CHI3L1 levels have been associated with inflammatory conditions including asthma, rheumatoid arthritis, and cancer, making it a candidate biomarker for monitoring disease progression and treatment response.

The assay has a reported detection range of 31.2-2000pg/mL and a sensitivity of 12pg/mL. Reported precision values include an intra-assay CV of 5.2% and an inter-assay CV of 9.6%. Linearity has been characterised in serum, EDTA plasma, and heparin plasma across dilution factors of 1:2, 1:4, 1:8, and 1:16, with serum recovery averaging 103% (range 97-109%) and plasma recovery averaging 105% (range 99-111%).

Suitable sample types include serum, plasma, tissue homogenates, cell culture supernatants, and other biological fluids, and the assay is specific for both natural and recombinant human CHI3L1. The target protein is a carbohydrate-binding lectin with a preference for chitin but lacks chitinase activity, and corresponds to UniProt entry P36222. The product is for research use only.