Human CHID1/Chitinase domain-containing protein 1 ELISA Kit

From Assay Genie, this competitive ELISA kit is designed for the precise detection of human CHID1 (Chitinase Domain-Containing Protein 1), also known as Stabilin-1-interacting chitinase-like protein (SI-CLP; Uniprot Q9BWS9). The assay uses a colorimetric, antigen-coated competitive format and is intended for use with serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples of human origin, falling within the immunology research area. Reported performance characteristics include a sensitivity of 0.188 ng/mL and a working range of 0.313-20 ng/mL, with absorbance read at 450 nm. CHID1 is a chitinase domain-containing protein implicated in immune response, inflammation, and tissue remodeling, and has been associated with conditions such as asthma, allergic diseases, and inflammatory disorders, making it a useful biomarker for related investigations. The procedure involves equilibration of TMB substrate, binding of sample with a biotin-labeled antibody at 37°C, washing, color development in the dark, and a stop reaction that changes the well color from blue to yellow. The kit is supplied sealed and should be stored at 2-8°C without cryopreservation. Validated linearity and recovery data are provided for serum, EDTA plasma, and heparin plasma samples across multiple dilutions.