Human Chemokine C-X-C-Motif Receptor 3 (CXCR3) ELISA Kit

C-X-C motif chemokine receptor 3 (CXCR3) plays a central role in directing activated T lymphocytes and natural killer cells toward sites of inflammation, and the Assay Genie CXCR3 ELISA Kit enables quantitative measurement of this receptor in human-derived specimens using a microplate-based immunoassay format. CXCR3 is a seven-transmembrane G protein-coupled receptor that mediates signaling by the interferon-inducible chemokines CXCL9 (MIG), CXCL10 (IP-10), and CXCL11 (I-TAC), driving migration of CD4+ Th1 lymphocytes, CD8+ cytotoxic T cells, and NK cells into inflamed or infected tissue.

Researchers apply this kit to characterize Th1-skewed immune responses in autoimmune and chronic inflammatory conditions, to profile tumor-infiltrating lymphocytes in oncology studies, and to monitor chemokine pathway activity in viral infection models including hepatitis C and HIV. The receptor's preferential expression on effector lymphocytes makes its circulating levels a useful biomarker for distinguishing Th1-driven pathology from Th2- or regulatory-mediated disease states, and pairing soluble CXCR3 measurements with its cognate ligands helps dissect interferon-γ axis activity in patient cohorts.

This human reactivity ELISA is supplied as a kit of 96 assays compatible with tissue homogenates, cell culture supernatants, and serum- or plasma-derived preparations in research use only workflows. Each plate includes pre-coated wells, matched detection antibody, biotin conjugate, and standard curve reagents sufficient to generate a quantitative readout when paired with appropriate sample dilution and a compatible microplate reader. Unopened kit components should be stored refrigerated and protected from light until first use, with reconstituted working solutions handled according to the supplied protocol insert.

For laboratories investigating T-cell trafficking, graft rejection, melanoma or colorectal cancer immunology, or the contribution of interferon-γ axis signaling to disease progression, the Assay Genie kit provides a reproducible tool for measuring soluble CXCR3 alongside its chemokine ligands in matched sample sets, supporting biomarker discovery and translational immunology programs.