Human CFL2/Cofilin 2 ELISA Kit

An ELISA Kit from Assay Genie is designed to measure human CFL2 (Cofilin-2) in serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. Detection is colorimetric, using a double-antibody sandwich format, and the kit is reactive with human specimens. Reported performance characteristics include a sensitivity of 9.375 pg/mL and a working range of 15.625–1000 pg/mL. The kit is intended for storage at 2–8°C for 12 months and is grouped under the Cell Biology research area.

Cofilin-2, also referenced by the synonyms CFL2, Cofilin-2 and Cofilin, muscle isoform, is described in the cited sources as a protein involved in actin dynamics, with a key role in cell migration and invasion. Dysregulation of cofilin-2 has been linked in the source material to cancer, cardiovascular diseases and neurodegenerative disorders.

Reported linearity values were 82–98% (1:2), 81–91% (1:4) and 85–101% (1:8) in serum (n=5); 84–101% (1:2), 83–96% (1:4) and 85–105% (1:8) in EDTA plasma (n=5); and 82–98% (1:2), 86–95% (1:4) and 94–104% (1:8) in heparin plasma (n=5). Reported recovery values were 86–100% (average 95%) in serum (n=5), 90–105% (average 97%) in EDTA plasma (n=5) and 85–105% (average 94%) in heparin plasma (n=5).